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active raf1 ee  (Sino Biological)


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    Structured Review

    Sino Biological active raf1 ee
    Active Raf1 Ee, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/raf1+(ee)%2C+active/RAF1+(EE)%2C+Active/pmc09986241-233-7-38
    Average 91 stars, based on 3 article reviews
    active raf1 ee - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Incubation:

    Article Title: Replication Study: RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth
    Article Snippet: .. The following kinase preparations were incubated in assay buffer (25 mM HEPES, pH 7.4, 10 mM MgCl 2 , 0.01% (v/v) Triton X-100, and 2 mM DTT) for 1 hr at room temperature: 500 nM (170 nM was used on some repeats to conserve material) of recombinant glutathione S-transferease (GST)-CRAF (Y340E/Y341E) kinase domain (sequence: 306-end) (SignalChem, cat# R01-13G); His-BRAF kinase domain (sequence: 417-end); and/or His-BRAF(V600E) kinase domain (sequence: 417-end) in the presence or absence of a fixed concentration of compound or vehicle (0.25% (v/v) DMSO). .. PLX4720 (Symansis, cat# SY-PLX4720), GDC-0879 (Selleckchem, cat# S1104), and Adenylylmethylenediphosphonate (AMP-PCP) disodium salt (Sigma-Aldrich, cat# M7510) were diluted in DMSO for their use.

    Recombinant:

    Article Title: Replication Study: RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth
    Article Snippet: .. The following kinase preparations were incubated in assay buffer (25 mM HEPES, pH 7.4, 10 mM MgCl 2 , 0.01% (v/v) Triton X-100, and 2 mM DTT) for 1 hr at room temperature: 500 nM (170 nM was used on some repeats to conserve material) of recombinant glutathione S-transferease (GST)-CRAF (Y340E/Y341E) kinase domain (sequence: 306-end) (SignalChem, cat# R01-13G); His-BRAF kinase domain (sequence: 417-end); and/or His-BRAF(V600E) kinase domain (sequence: 417-end) in the presence or absence of a fixed concentration of compound or vehicle (0.25% (v/v) DMSO). .. PLX4720 (Symansis, cat# SY-PLX4720), GDC-0879 (Selleckchem, cat# S1104), and Adenylylmethylenediphosphonate (AMP-PCP) disodium salt (Sigma-Aldrich, cat# M7510) were diluted in DMSO for their use.

    Article Title: MAST1 drives cisplatin resistance in human cancers by rewiring cRaf independent MEK activation
    Article Snippet: Recombinant active MEK1 for MST , Proqinase , Cat#0550–0000-3. .. Recombinant active c-Raf , SignalChem , Cat#R01–13G. .. Recombinant inactive ERK2 , SignalChem , Cat#M28–14G.

    Sequencing:

    Article Title: Replication Study: RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth
    Article Snippet: .. The following kinase preparations were incubated in assay buffer (25 mM HEPES, pH 7.4, 10 mM MgCl 2 , 0.01% (v/v) Triton X-100, and 2 mM DTT) for 1 hr at room temperature: 500 nM (170 nM was used on some repeats to conserve material) of recombinant glutathione S-transferease (GST)-CRAF (Y340E/Y341E) kinase domain (sequence: 306-end) (SignalChem, cat# R01-13G); His-BRAF kinase domain (sequence: 417-end); and/or His-BRAF(V600E) kinase domain (sequence: 417-end) in the presence or absence of a fixed concentration of compound or vehicle (0.25% (v/v) DMSO). .. PLX4720 (Symansis, cat# SY-PLX4720), GDC-0879 (Selleckchem, cat# S1104), and Adenylylmethylenediphosphonate (AMP-PCP) disodium salt (Sigma-Aldrich, cat# M7510) were diluted in DMSO for their use.

    Concentration Assay:

    Article Title: Replication Study: RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth
    Article Snippet: .. The following kinase preparations were incubated in assay buffer (25 mM HEPES, pH 7.4, 10 mM MgCl 2 , 0.01% (v/v) Triton X-100, and 2 mM DTT) for 1 hr at room temperature: 500 nM (170 nM was used on some repeats to conserve material) of recombinant glutathione S-transferease (GST)-CRAF (Y340E/Y341E) kinase domain (sequence: 306-end) (SignalChem, cat# R01-13G); His-BRAF kinase domain (sequence: 417-end); and/or His-BRAF(V600E) kinase domain (sequence: 417-end) in the presence or absence of a fixed concentration of compound or vehicle (0.25% (v/v) DMSO). .. PLX4720 (Symansis, cat# SY-PLX4720), GDC-0879 (Selleckchem, cat# S1104), and Adenylylmethylenediphosphonate (AMP-PCP) disodium salt (Sigma-Aldrich, cat# M7510) were diluted in DMSO for their use.

    other:

    Article Title: Erianin suppresses constitutive activation of MAPK signaling pathway by inhibition of CRAF and MEK1/2
    Article Snippet: Active BRAF (#B08-11BG), active BRAF V600E (#B08-12G), active RAF1 (EE) (#R01-13G), corresponding RAF substrate inactive MEK1 (#M02-14BG), as well as active MEK1 (#M02-10G), active MEK2 (#M03-10G), inactive ERK1 protein (#M29-14G), and inactive ERK2 protein (#M28-14U) were obtained from Signal Chem.



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    Image Search Results


    KEY RESOURCES TABLE

    Journal: Cancer cell

    Article Title: MAST1 drives cisplatin resistance in human cancers by rewiring cRaf independent MEK activation

    doi: 10.1016/j.ccell.2018.06.012

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Recombinant active c-Raf , SignalChem , Cat#R01–13G.

    Techniques: Recombinant, Negative Control, Viability Assay, Kinase Assay, Extraction, Ab Array, Activation Assay, Enzyme-linked Immunosorbent Assay, Reverse Transcription, shRNA, Sequencing, Plasmid Preparation, Software